Four Drosophila heat shock genes at 67B: characterization of recombinant plasmids
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چکیده
منابع مشابه
Four Drosophila heat shock genes at 67B: characterization of recombinant plasmids.
A 33 kilobase region of the 67B locus of Drosophila melanogaster genome has been isolated. The genes for the 27K, 26K, 23K and 22K heat shock induced proteins are contained within an 11 kb segment. the 27K gene, approximately 1.4 kb in length, and the 23K gene, approximately 1.0 kb in length, are separated by about 1.5 kb of spacer DNA; a third block of transcription, which encodes the 26K gene...
متن کاملSequence homologies in the 5' regions of four Drosophila heat-shock genes.
We report nucleotide sequences of the regions surrounding the 5' ends of the genes for Drosophila melanogaster heat-shock proteins hsp83, hsp68, and hsp26, located at chromosome positions 63BC, 95D, and 67B, respectively. As in other eukaryotic genes, the sequence T-A-T-A-A-A-A-T occurs about 30 nucleotides upstream from the sites of mRNA initiation. Three additional sequence homologies and a d...
متن کاملBinding of heat shock factor to and transcriptional activation of heat shock genes in Drosophila.
Heat shock factor (HSF) binds to heat shock elements (HSEs) and the binding can be highly cooperative. Here we report an analysis of binding of Drosophila HSF to both native and synthetic heat shock regulatory regions. We find that cooperative binding of HSF requires close proximity, rather than helical alignment, of HSEs. Two or more trimeric HSEs organized as contiguous 5 bp units show much h...
متن کاملActivation of the major drosophila heat-shock genes in vitro.
Inactive Drosophila heat-shock genes of isolated diploid nuclei can be induced to a transcriptionally active state by exposure to cytoplasmic extracts from heat-shocked Drosophila cells. No effect was observed on histone gene transcription, and extracts from non-heat-shocked cells were ineffective. The factor in the cytoplasmic extract has been partially purified and characterized. It is protea...
متن کاملTranscription of the major Drosophila heat-shock genes in vitro.
Active eukaryotic genes are more accessible to some proteins than bind DNA than are inactive genes. In order to probe the accessibility of the Drosophila heat-shock genes we have isolated nuclei from Drosophila tissue culture cells and have used these nuclei as templates for Escherichia coli RNA polymerase. With nuclei isolated from cells that had not been heat shocked, the synthesis of heat-sh...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1980
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/8.19.4441